1) chromatin immunoprecipitaion (ChIP)
染色质免疫沉淀(ChIP)
2) chromatin immunoprecipitation assay (ChIP)
染色质免疫沉淀技术(ChIP)
3) Chromatin immunoprecipitation
染色质免疫沉淀
1.
AIM: To construct a new method named PolII-ChIP-PE- MS (RNA polymerase II-Chromatin immunoprecipitation- primer extension-mass spectrum), and to validate the reliability of this method in the study of single nucleotide polymorphism (SNP) allele-specific quantification o RNA polymerase loading.
目的:建立RNA多聚酶Ⅱ-染色质免疫沉淀-引物延伸-飞行时间质谱技术(PolⅡ-ChIP-PE-MS),并利用该方法检测SNP等位特异磷酸化PolⅡ结合量,为活体状态下研究疾病关联基因SNP的功能研究提供一种高通量的研究手段。
2.
After the cells were cross-linked by formaldehyde and chromatin was sonicated by ultrasonic, we performed RNA polymerase Ⅱ-chromatin immunoprecipitations (PolⅡ-ChIP) to precipitate the promoter sequences and validated them by PCR.
方法常规培养和刺激HepG2和L02细胞,甲醛固定细胞,超声剪切染色质,采用利用PolⅡ CTD末端Ser5特异性抗体进行染色质免疫沉淀(PolⅡ-ChIP),针对文献报道的8个可能启动子区设计引物进行PCR扩增,鉴定ESR1基因启动子的使用情况。
3.
The binding of Ezh2 and H3K27me3 on the Ngn 1 promoter was analyzed using chromatin immunoprecipitation assay.
方法通过Western印迹检测全反式维甲酸(RA)诱导TuJ1蛋白的表达;通过染色质免疫沉淀技术检测Ngn1基因启动子区Ezh2的结合与H3K27三甲基化的变化;通过实时定量RT-PCR检测RA诱导P19细胞分化后Ezh2mRNA表达。
4) chromatin immunoprecipitation assay
染色质免疫沉淀技术
1.
Objective To establish a Chromatin immunoprecipitation assay to analyze transcriptional regulation of gene expression in hepatocytes.
目的建立一种染色质免疫沉淀技术(ChIP)分析肝细胞基因表达的转录调控方法。
2.
Now, chromatin immunoprecipitation assay (ChIP) is ideally suited for investigating DNA-protein interactions in vivo.
染色质免疫沉淀技术(chromatin immunoprecipitation assay,简称ChIP)是目前唯一研究体内DNA与蛋白质相互作用的方法。
3.
Nowadays, in comparison with other methods, chromatin immunoprecipitation assay (ChIP) is ideally suited for studying DNA-protein interactions in vivo.
与其他方法相比,染色质免疫沉淀技术(chromatin immunoprecipitation assay,ChIP)是一种近来研究体内DNA与蛋白质相互作用的最好方法之一。
5) Chromatin immunoprecipitation
染色质免疫共沉淀
1.
Here we adopted Chromatin immunoprecipitation linked to microarrays(ChIP-chip),for the first time,to profile the variations in H3K9me3 in CpG island regions in squamous carcinomas of the cervix patients and controls\' ChIP-qPCR is used to validate the microarray results.
方法:收集宫颈鳞癌组织5例及对照组织5例,采用染色质免疫共沉淀联合芯片技术(ChiP-chip)在全基因组范围内对宫颈鳞癌组织及正常宫颈组织中的组蛋白H3K9me3进行高通量的筛选,获得宫颈鳞癌组织中H3K9me3的表达图谱。
6) chromatin immunoprecipitation(ChIP) assay
染色质免疫沉淀实验
补充资料：放射免疫沉淀反应
放射免疫沉淀反应
是用放射性同位素标记的抗体或抗原进行免疫沉淀反应的一种方法。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条