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1)  real time RT PCR
实时荧光定量聚合酶链反应法
2)  real-time reverse transcription polymerase chain reaction
实时荧光定量反转录聚合酶链反应
1.
Methods The expression of PBMC IP-10 mRNA in 42 children with KD and 40 healthy children were assayed by real-time reverse transcription polymerase chain reaction(real-time RT-PCR).
方法实时荧光定量反转录聚合酶链反应(real-time RT-PCR)检测42例KD及40例健康儿童PBMC中IP-10 mRNA表达。
3)  real-time fluorescence reverse transcription polymerase chain reaction (RT-PC
荧光实时定量反转录-聚合酶链反应
4)  real-time polymerase chain reaction
实时荧光定量聚合酶链反应
1.
Methods DNASE1 mRNA expression was detected by real-time polymerase chain reaction and its alternatively spliced transcripts were performed by capillary electrophoresis.
 方法 以实时荧光定量聚合酶链反应 (real- time PCR)方法检测 DNASE1的 m RNA表达水平 ,以毛细管电泳技术分析 m RNA编码区替代剪接体 ,结合单核苷酸多态性 (single nucleotide polymorphisms,SNPs)单倍型了解基因结构对表达的影响。
5)  real-time fluorescence relative quantitative RT-PCR
实时荧光定量-逆转录-聚合酶链反应
6)  real time quantitative RT-PCR
实时荧光定量逆转录聚合酶链反应
1.
Methods Adult Wistar rats were exposed to hypobaric hypoxic environment for 2 hours following which the expression of AngⅡwere observed at 3 hours,24 hours,3 days,7 days and 14 days with real time quantitative RT-PCR and Western-blotting,respectively.
L-1O2)2h后,分别在3h、24h、3d、7d和14d,通过实时荧光定量逆转录聚合酶链反应、蛋白免疫印迹法检测AngⅡ在视网膜中的表达情况。
补充资料:聚合酶链反应


聚合酶链反应
polymerase chain reaction,PCR

是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA)片断大量增扩,达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体,因其假阳性率较高,故应谨慎使用。
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