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1)  T-DNA insertional library Directed by:prof. LIU Zhengping
T-DNA插入诱变
2)  T-DNA insertional mutagenesis
T-DNA插入突变
1.
To elucidate the molecular mechanism of pathogenicity in Colletotrichum lagenarium,the casual agent of cucumber anthracnose,conditions for T-DNA insertional mutagenesis using Agrobacterium-mediated transformation approach were established and optimized.
建立并优化了农杆菌介导转化瓜类炭疽菌(Colletotrichum lagenarium)获得T-DNA插入突变体体系,包括农杆菌使用浓度、农杆菌与炭疽菌孢子共培养时间、抗生素配合使用抑制农杆菌污染等。
2.
Therefore, based on the T-DNA insertional mutagenesis of M, grisea in our lab, we screened for mutants with stable mut.
本研究在本实验室创建的T-DNA插入突变体库的基础上,筛选性状稳定的致病相关突变体,深入分析突变体的表型,克隆和解析相关基因的生物学功能。
3.
Therefore, we studied on large scale T-DNA insertional mutagenesis of the fungus by Agrobacterium fumefaciens-mediated tr.
为此,本研究利用农杆菌介导转化T—DNA插入技术,大规模创建稻瘟病菌T-DNA插入突变体,并对部分突变体进行了分析,取得了如下结果: 在Mullins等建立的农杆菌转化Fusarium方法基础上,进一步优化了农杆菌介导转化稻瘟病菌获得T-DNA插入突变的条件,包括选择转化子的潮霉素B用量、抑制农杆菌的抗生素头孢噻肟钠和羧苄青霉素的配比、不同转化阶段培养基的选择等。
3)  T-DNA insertion mutant
T-DNA插入突变体
1.
Besides, T-DNA insertion mutant lines of pollen surface proteins have been purchased, and through T-DNA insertion and locus analysis, 96 homozygous mutant lines have been acquired.
此外,购买了花粉表面蛋白质T-DNA插入突变体,通过分子生物学方法进行了T-DNA插入鉴定以及插入位点鉴定,并获得了96个突变体株系的纯合植株。
4)  T-DNA insertion
T-DNA插入
1.
Effection of pigments and the protein complexes of thylakoid membrane in Hcf-1 mutant of Arabidopsis by T-DNA insertion;
T-DNA插入对拟南芥突变体色素和内囊体膜色素蛋白复合物的影响
2.
A disease lesion mimic mutant named AZT91 was screened from T-DNA insertion mutant of rice.
从T-DNA插入突变体中筛选到一个类病斑突变体AZT91,主要表现为生长缓慢、植株矮小、叶片出现条状褐斑,最后死亡。
3.
In this study,an albino mutant caused by T-DNA insertion in rice was identified.
Basta抗性检测、PCR分子检测及Southern杂交证实,该突变体是由单一T-DNA插入所引起的,白化苗性状与T-DNA共分离。
5)  insertional mutagensis
插入诱变
6)  a line with T-DNA copies inserted independently
双拷贝T-DNA插入
补充资料:插入诱变
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CAS号:

性质:又称插入突变(insertional mutation)。DNA链上由于插入额外的核苷酸或DNA片段而引起的突变。 

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