1)  cDNA
噬菌体表面展示文库
1.
Objective: Identify the human hepatocarcinoma cell strain with the highest reactivity to hALR and construct the phage-displayed library with its cDNA as the basis of the selection of special protein binding hALR from this library.
方法:以~3H-TdR掺入法比较hALR对不同肝癌细胞株促增殖作用的大小,从数株中鉴定出一株对hALR刺激具有高反应性的细胞株,以Promega公司PolyATtract System 1000试剂盒通过磁珠分离一步法提取肝癌细胞株的mRNA,然后利用Novagen公司的T7Select10-3 OrientExpress cDNA Cloning System,Random Primer试剂盒构建出肝癌细胞cDNA的噬菌体表面展示文库,通过噬菌体滴度分析和PCR评价cDNA文库质量。
2)  Phagocytosis
噬菌
3)  bacteriophage
噬菌体
1.
Methods for preventing the contamination of bacteriophage in the processing of fermented yogurt;
酸奶发酵过程中防止噬菌体感染的措施
2.
Methods for protecting bacteriophage in dairy industry;
危害乳业的噬菌体的预防对策
3.
Isolation and Characterization of 4 Bacteriophages Strains in Yoghurt;
4株酸奶噬菌体的分离及其生物学特性
4)  Phage
噬菌体
1.
Study on Pollution of Phage in Yoghurt Processing;
酸奶生产中的噬菌体污染
2.
The Selection of the Antiphage Strain by Streptomyces Erythreus;
红霉素链霉菌抗噬菌体菌株的选育
3.
Selection of phage resistant strains from 2 keto D gluconic acid producing strain;
2-酮基-D-葡萄糖酸产生菌荧光假单胞菌K1005抗噬菌体菌株的选育
5)  somatic coliphages
SC噬菌体
1.
The concentrations of somatic coliphages(SC) and F-specific RNA bacteriophages in effluent of three wastewater treatment plants in Beijing city were detected.
测定了北京市3座城市污水处理厂各处理单元出水中的SC噬菌体和F-RNA噬菌体浓度。
2.
The concentrations of microorganism indicators(faecal coliforms,somatic coliphages and F-specific bacteriophages) in five wastewater reclamation systems with different treatment processes were studied.
选取5个采用不同处理工艺的小区生活污水再生处理系统,检测了各小区生活污水和再生水中粪大肠菌、SC噬菌体及F-噬菌体的浓度水平。
3.
The concentration of three pathogenic microbial indicators(fecal coliforms,somatic coliphages and F-specific bacteriophages) in a few kinds of wastewater were detected,also the detection precision was analyzed.
对多种污水样品中的粪大肠菌、SC噬菌体(somatic coliphage)和F-噬菌体(F-spe-cific bacteriaphages)三种病原性指示微生物的浓度进行了测定,对检测结果进行了精度分析,结果表明:三种指示微生物浓度检测结果的相对偏差频数分布均呈正态,同一水样平行检测结果的相关系数都很高,相对偏差均有随浓度升高而降低的趋势。
6)  F-specific bacteriophages
F-噬菌体
1.
The concentrations of microorganism indicators(faecal coliforms,somatic coliphages and F-specific bacteriophages) in five wastewater reclamation systems with different treatment processes were studied.
选取5个采用不同处理工艺的小区生活污水再生处理系统,检测了各小区生活污水和再生水中粪大肠菌、SC噬菌体及F-噬菌体的浓度水平。
2.
The concentration of three pathogenic microbial indicators(fecal coliforms,somatic coliphages and F-specific bacteriophages) in a few kinds of wastewater were detected,also the detection precision was analyzed.
对多种污水样品中的粪大肠菌、SC噬菌体(somatic coliphage)和F-噬菌体(F-spe-cific bacteriaphages)三种病原性指示微生物的浓度进行了测定,对检测结果进行了精度分析,结果表明:三种指示微生物浓度检测结果的相对偏差频数分布均呈正态,同一水样平行检测结果的相关系数都很高,相对偏差均有随浓度升高而降低的趋势。
参考词条
补充资料:噬菌体展示库

噬菌体展示库(phage display library)的基本原理是将外源基因同丝状噬菌体fd或mb的外壳蛋白p8基因或p3基因融合后导入噬菌体基因组,表达产生的外源肽与外壳蛋白p8或p3形成融合蛋白,展示在噬菌体的表面。导入了各种各样外源基因的一群噬菌体,就构成一个展示各种各样外源肽的噬菌体展示库。当用一个蛋白质去筛查一个噬菌体展示库时,就会选择性地同与其有相互作用的某个外源肽相结合,从而分离出展示库里的某个特定的噬菌体,研究该噬菌体所含外源基因的生物学功能。 基于同一原理的另一种实验系统是将编码细菌表面的菌毛蛋白的基因,同外源基因融合,转入细菌细胞后表达,在细菌表面的菌毛中出现融合蛋白。当遇到能同外源基因的蛋白质产物相互作用的蛋白质时就能选择性地与融合蛋白相结合。这样,也就起到展示库的作用,可以筛选有相互作用的蛋白质。

说明:补充资料仅用于学习参考,请勿用于其它任何用途。