1) FQ-RT-PCR
荧光定量逆转录聚合酶链反应
1.
Objective To develop fluorescence quantitative reverse transcription polymerase chain reaction (FQ-RT-PCR) for quantification of iASPP mRNA, we used TaqMan probe and detected its level in hepatocellular carcinoma (HCC), adjacent liver tissue ,normal liver tissue and hepatoma cell lines (HepG2 and SMMC-7721 ).
目的 采用TaqMan探针技术,建立实时荧光定量逆转录聚合酶链反应(FQ-RT-PCR),应用该方法检测iASPP mRNA在肝癌及其癌旁组织、正常肝组织中以及人肝癌细胞株HepG2、SMMC-7721中的表达情况,分析iASPP与肝癌的相关关系,探讨iASPP在肝细胞癌发生发展中的可能作用及其临床意义。
2.
MethodsFQ-RT-PCR is one of the advanced technologies in gene quantity investigation.
方法： 荧光定量逆转录聚合酶链反应(FQ-RT-PCR)方法。
3.
Objective ①To develop fluorescence quantitative reverse transcription polymerase chain reaction (FQ-RT-PCR) for quantitating DEAH (Asp-Glu-Ala-His) box polypeptide 32 (DHX32) mRNA, FLICE-like inhibitory protein long form (FLIP(L)) mRNA and FLICE-like inhibitory protein short form (FLIP(S)) mRNA.
目的 ①建立检测DHX32 mRNA、FLIP(L) mRNA以及FLIP(S) mRNA实时荧光定量逆转录聚合酶链反应(FQ-RT-PCR)方法。
2) Fluorescence quantitative nested reverse transcripate PCR
荧光定量套式逆转录酶聚合酶链反应
3) Fluorescence Quantitative nested Reverse Transcriptase PCR
荧光定量巢式逆转录酶聚合酶链反应
4) real-time fluorescence relative quantitative RT-PCR
实时荧光定量-逆转录-聚合酶链反应
5) real time quantitative RT-PCR
实时荧光定量逆转录聚合酶链反应
1.
Methods Adult Wistar rats were exposed to hypobaric hypoxic environment for 2 hours following which the expression of AngⅡwere observed at 3 hours,24 hours,3 days,7 days and 14 days with real time quantitative RT-PCR and Western-blotting,respectively.
L-1O2)2h后,分别在3h、24h、3d、7d和14d,通过实时荧光定量逆转录聚合酶链反应、蛋白免疫印迹法检测AngⅡ在视网膜中的表达情况。
6) real-time quantitative reverse transcription polymerase chain reaction
实时荧光定量逆转录-聚合酶链反应
1.
The COL3A1 mRNA was measured by real-time quantitative reverse transcription polymerase chain reaction(RT-PCR).
方法应用基因芯片技术和实时荧光定量逆转录-聚合酶链反应(RT-PCR)技术,检测Ⅲ型胶原α链在不同舌苔中的表达水平。
补充资料：聚合酶链反应
聚合酶链反应
polymerase chain reaction,PCR
是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA）片断大量增扩，达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体，因其假阳性率较高，故应谨慎使用。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条