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1)  His-Tag affinity purification
His-Tag亲和纯化
2)  His-Tag purification
His-Tag纯化
3)  His-tag
亲和标记His-tag
1.
Objective:To construct the recombinant protein DAB389 (Gly4Ser)2-αMSH containing His-tag in order to simplify purification.
目的:构建含有亲和标记His-tag的DAB389(Gly4Ser)2-αMSH重组蛋白,便于融合蛋白质的纯化。
4)  His-tag affinity
His-tag蛋白纯化
5)  affinity purification
亲和纯化
1.
Biomimetic affinity purification of recombinant human lactoferrin from the milk of transgenic cows
转基因牛乳中重组人乳铁蛋白的仿生亲和纯化
2.
The rabbits were immunizedand with the purified antigens,and the serum were collected and purified by affinity purification.
方法:构建表达GST-ANKRD17C端融合蛋白的质粒,在大肠杆菌中诱导表达;制备GST-ANKRD17C端抗原融合蛋白后免疫家兔,对获得的兔多克隆抗血清进行亲和纯化;纯化后的抗体经过Westernblot鉴定,用于细胞免疫荧光染色检测。
3.
Methods:The proteins which interacted with gankyrin were screened and analyzed by affinity purification technology and mass spectrometry.
方法:采用亲和纯化技术及质谱鉴定筛选与gankyrin相互作用的蛋白质。
6)  Affinity chromatography
亲和纯化
1.
hydrophila ZN1 strain by affinity chromatography with McAb 3C12B11 and eluated with glycine, and its hemolytic activity was analyzed through hemolytic reactions with 1% suspension of erythrocytes of Anguilla anguilla, rabbits and sheep.
目的用亲和纯化法纯化嗜水气单胞菌气溶素,从单因子水平分析嗜水气单胞菌的溶血特性。
补充资料:Tag flash point test
分子式:
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性质:测定物质受热产生的可燃气体与空气的混合物接触火焰时发生闪火的最低温度的试验,即为闪点试验。按照所用仪器的不同,又分为开口法和闭口法两种。开口法适用于闪点较高如150℃左右,甚至高达300℃的石油产品,如润滑油、深色石油、增塑剂等,闭口式适用于闪点稍低的产品。泰格闪点试验,是用泰格公司的闪点试验仪进行的闪点测定试验。

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