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1)  phosphotase digestion
磷酸酶消化法
1.
(2)By phosphotase digestion,the relationship between phosphorylation and TAL activity was determined.
(2)对上述不同血清浓度培养2、4、8h细胞提取总蛋白采用磷酸酶消化法,检测消化前后TAL活性。
2)  Pyrophosphoric acid method
焦磷酸消化法
3)  phosphorylase phosphatase
磷酸化酶磷酸酶
4)  phosphorylase [英]['fɔsfəri,leis]  [美]['fɑsfərə,les, -,lez]
磷酸化酶
1.
Clinical significance of determination of thymidine phosphorylase in therapy evaluation of capecitabine for colorectal cancer;
胸腺嘧啶磷酸化酶的表达与卡培他滨治疗大肠癌疗效评价的临床意义
2.
Objective To investigate theoretically the effect of Ca 2+ oscillations on a particular example of cell regulation: the phosphorylation-dephosphorylation cycle controlling the activation of glycogen phosphorylase in hepatocytes.
肝细胞中的磷酸化 去磷酸化动力学过程 ,在Ca2 + 振荡的调控下 ,其糖原磷酸化酶的浓度呈现同频、同步振荡。
5)  Phosphatase [英]['fɔsfəteis]  [美]['fɑsfə,tes, -,tez]
磷酸化酶
1.
Inorganic Phosphorus and Its Correlation with Soil Phosphatase and Phosphorus-solubilizing Microbes in the Main Soils of Shandong Province;
山东省主要土类的无机磷及其与磷酸化酶和解磷微生物的关系
2.
Phosphatase Activities of Main Soil Types in Shandong Province and their Relationship with Soil P Releasing Properties;
山东主要土类中磷酸化酶及其与供磷特性的关系
6)  enzyme digestion
酶消化法
1.
METHODS:Freshly extracted human permanent teeth were collected and sectioned under cooling ECS solution and then the odontoblasts were isolated through enzyme digestion.
方法:选取新鲜拔除的健康恒牙,利用牙科专用慢速切锯制备牙齿切片,采用酶消化法分离出完整的人成牙本质细胞,利用RT-PCR技术直接从人成牙本质细胞中克隆L型钙离子通道α1亚基D亚型特异性基因片段,将其亚克隆入pGM-T载体进行序列测定。
2.
Methods The sertoli cells in rats testicular were obtained through multiple enzyme digestion and these cells were cultivated in vitro to maintain their favorable activities.
方法利用多重酶消化法,获取睾丸Sertoli细胞,进行体外原代培养以维持其良好的活性。
3.
Results:The HOBs purified by enzyme digestion in vitro had a normal function showing synthesized alkaline phosphatase(ALP)and forming calcified nodules as observed in vivo,and significant difference in optical density(OD490) was found between morphine group with different concentrations and the control group(P<0.
方法:用胰酶-胶原酶消化法从正常女性松质骨中分离培养成骨细胞,用茜素红染色法、改良Gomonri钙钴法鉴定成骨细胞;制备不同浓度的吗啡及其拮抗剂纳洛酮,作用于体外培养的成人正常成骨细胞上,通过四甲基偶氮唑盐(MTT)比色法检测吗啡对其的影响;通过RT-PCR法半定量分析不同浓度的吗啡及其拮抗剂纳洛酮对成骨细胞骨钙素(BGP mRNA)表达的影响。
补充资料:磷酸化酶磷酸酶
分子式:
CAS号:

性质:又称磷酸化酶磷酸酯酶。磷酸酯酶一种,EC3.1.3.17,分子量5×104。它的底物是有活性的磷酸化酶a。它所催化的反应是磷酸化酶a一级结构中14序号上的丝氨酸上与磷酸基相连接酯键水解(断裂)而游离出4个无机磷酸分子,使磷酸化酶a转变为无活性的磷酸化酶b(变构效应物,即AMP可使它成为有活性的酶)。它的作用正好与在ATP(腺苷三磷酸)存在下的磷酸化酶激酶(phosphorylase kinaseEC2.7.1.38.)的作用相反。它在糖原的生成和代谢中起催化和调节的作用。通常磷酸化酶磷酸酶可从兔肌肉组织中提取。

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