1) in vitro co-culture
体外共培养
1.
A basic in vitro co-culture system for ovine male germ cells has been established in this study.
本研究初步建立了绵羊雄性生殖细胞体外共培养系统,同时为从分子水平对共培养系统中各级生殖细胞的存活进行检测,首次成功克隆了绵羊各级生殖细胞的特异标记基因,如E型钙粘连蛋白基因(E-cadherin gene,cdh1)、联会复合体蛋白3基因(synaptonemal complex protein-3 gene,scp3)、过渡蛋白1基因(Transition protein-1 gene,tnp1)和过渡蛋白2基因(Transition protein-2 gene,tnp2),并利用得到的cDNA序列进行引物设计,用于转录表达分析来鉴定绵羊生殖细胞共培养系统中的各级生殖细胞。
2) coculture
共培养体
1.
In order to research into its physiological and ecological characteristics and to lay the scientific basis for the development of highly efficient anaerobic biological treatment technology,the syntrophic acetogen cocultures were enriched and Selective bred by the Selective medium which is mixed by the Propionic acid and butyric acid from the anaerobic activated sludge with methanogens.
基于以上认识和研究现状,本论文开展了产氢产乙酸互营共培养体的分离筛选工作,并以此为基础,对所筛选的共培养体进行了生理生态特性研究,并进一步探索了以其强化厌氧生物处理系统效能的可行性。
2.
In order to study HPA\'s physiological and ecological characteristics and to establish the scientific basis for the development of highly efficient anaerobic biological treatment processes, the syntrophic acetogen cocultures are enriched and selectively bred from the anaerobic activated sludge with methanogens by selective medium, using propionic and butyric acids as the only carbon source.
为了深入了解其生理生态特性,为开发高效的厌氧生物处理技术提供理论基础,采用丙酸和丁酸混合培养基,以具有甲烷发酵功能的厌氧活性污泥为出发菌群,进行了产氢产乙酸菌互营共培养体的选育,并借助于PCR-DGGE技术对其菌群结构进行了解析。
3) culture in vitro
体外培养
1.
Improvement on culture in vitro of rat cerebral cortical astrocytes;
大鼠大脑皮层星形胶质细胞体外培养方法改良
2.
Progress in research on embryo culture in vitro;
胚胎体外培养的研究进展
3.
Mouse cardiomyocytes culture in vitro;
小鼠心肌细胞的体外培养
4) in vitro
体外培养
1.
Improvement of cultural method in vitro for Echinococcus multilocularis metacestodes and primary identification of culture product;
泡球蚴体外培养方法的改进及培养产物的初步鉴定
2.
The Modulation of Endochondral Ossification in Rat Nasal Chondrocyte Cultures with 45S5 in vitro;
固骼生对体外培养鼠鼻中隔软骨细胞增殖及骨化的初步研究
3.
Effects of DSPP antisense oligodeoxynucleotide on development and mineralization of mouse molars in vitro;
牙本质涎磷蛋白反义核酸对体外培养牙胚发育、矿化的影响
5) culture
[英]['kʌltʃə(r)] [美]['kʌltʃɚ]
体外培养
1.
Spermatogonial stem cells transplantation and culture:a research progress;
精原干细胞移植及体外培养研究进展
2.
Isolation and Culture of Bovine Fetal Fibroblasts;
牛胎儿成纤维细胞分离与体外培养
3.
Human Bone Marrow Mesenchymal Stem Cell (BM-MSC) Culture and the Expression of Hematopoietic Cytokines;
人骨髓间充质干细胞体外培养及其细胞因子表达的研究
6) in vitro culture
体外培养
1.
Observation of the protective effects of OCE on retina tissue in vitro cultured;
OCE对体外培养的视网膜组织保护作用的观察
2.
Analysis of influence factors of in vitro fertilization laboratory quality control on in vitro culture of mouse embryos;
IVF实验室质控中影响鼠胚体外培养的因素分析
3.
Preliminary research on in vitro culture of human primordial germ cells;
人原始生殖细胞体外培养的初步研究
补充资料：共培养
共培养
按照一般体外授精、胚胎移植的常规，是将2～8分裂球阶段的胚胎转移到子宫腔内；而在自然妊娠过程，卵子和精子在输卵管壶腹部相遇，结合，受精卵在输卵管内分裂约3天后形成桑椹体才下降到子宫腔内。这也可能是体外受精、胚胎移植中成功率不高的原因。所谓共培养指采用人输卵管壶腹部上皮细胞，在HamF10或Earle培养液中培养6～7天形成结合—片的单层。在这样共培养条件下，78%胚胎可达到桑椹体阶段，69%可达到胚泡阶段。也可用输卵管上皮细胞飘流的培养液。另外，也有人应用成熟卵泡的颗粒细胞或子宫内膜细胞进行共培养，这些都在研究中。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条