2) normalized cDNA library
均一化cDNA文库
1.
Here we described the methods of ESTs preparation as well as the c onstruction of a normalized cDNA library and described EST s various application s.
本文介绍了EST的制备方法,以及构建均一化cDNA文库的方法,并介绍了EST在以上各方面的应用。
2.
In this study the total RNAs of maize hybrid Y22 ears that are during the important period -ear development period-were isolated using Trizol Reagent, and synthesis the cDNA strands,then construct the normalized cDNA library,which is the basis for the study genes about heterosis.
本工作通过Trizol Reagent分离了强优势玉米杂交种豫玉22关键发育时期—雌穗分化时期(生长锥伸长和小穗分化、小花分化、花丝始伸和果穗增长)—的总RNA,反转录合成cDNA,构建了均一化cDNA文库,为进一步研究和分离与杂种优势相关的基因构建了技术平台。
3) full-length cDNA library
全长cDNA文库
1.
Construction of full-length cDNA library of Candida albicans using SMART technology;
SMART技术构建白念珠菌全长cDNA文库
2.
Construction and analysis of a full-length cDNA library of Plutella xylostella in Fuzhou,China;
福州地区小菜蛾全长cDNA文库构建与分析
3.
Application of RACE method on the construction of full-length cDNA library for floral buds in Forsythia suspense
RACE法在连翘花蕾全长cDNA文库构建中的应用
4) full-length cDNA Plasmid Library
全长cDNA质粒文库
1.
To construct the full-length cDNA plasmid library for adult worms of Taenia solium and then to form a gene expression pattern in order to establish foundation for screening the genes used for vaccine development and preparation of diagnostic antigens.
方法提取猪带绦虫成虫mRNA,构建pBluescriptIISK全长cDNA质粒文库,测定扩增文库的滴度;用载体克隆位点两端的引物进行PCR扩增,以检测所构建文库的质量。
6) gonad development
线性化cDNA文库
1.
The construction of normalized cDNA library and preliminary EST analysis from the gonad development and sexual differentiation related organs of Scylla serrata
锯缘青蟹性腺发育与性别分化相关组织混合线性化cDNA文库构建及EST初步分析
补充资料:均一
1.见"均壹"。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
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