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1)  two-hybrid yeast
双杂交酵母
1.
The two-hybrid yeast system used had high sensitivity and could be used with the estrogenic agonistic and antagonistic activities to analyze the research on disrupt mechanism of xenobiotics.
采用重组人雌激素受体基因片段(hER)及共激活因子(GRIP1)双杂交酵母检测体系,检测9种酚类物质的类雌激素和抗雌激素效应。
2)  Yeast two-hybrid
酵母双杂交
1.
Screening of phenolic endocrine disrupting compounds with yeast two-hybrid system.;
酵母双杂交法筛检酚类物质的内分泌干扰作用
2.
Screening and identification of the interaction of STCH and RanBP9 by yeast two-hybrid system;
利用酵母双杂交技术筛选鉴定STCH与RanBP9的相互作用
3.
Screening the interaction proteins of MRP2 by yeast two-hybrid system;
应用酵母双杂交系统筛选MRP2胞内区相互作用蛋白
3)  yeast two hybrid
酵母双杂交
1.
Screening of the protein interacting with inhibitor of differentiation 1′ using yeast two hybrid method;
酵母双杂交筛选与分化抑制因子Id1′相互作用的蛋白
2.
The identification of protein interaction after yeast two hybrid of RIG-G gene;
RIG-G基因在酵母双杂交后的蛋白互作验证
3.
Objective To construct yeast two hybrid cDNA library of human brain in yeast cell Y187.
应用营养缺陷的方法在SD/-Leu平板上筛选并收集所有克隆从而获得酵母双杂交的人脑cDNA文库。
4)  yeast two-hybrid system
酵母双杂交
1.
Construction of the bait vector of HCV core in yeast two-hybrid system and amplification,purification,evaluation of the cDNA gene bank from human fetal liver;
丙型肝炎病毒核心蛋白酵母双杂交诱饵载体的构建及人胎肝cDNA文库的扩增、纯化和鉴定
2.
Role of HCV core protein in yeast two-hybrid system in reporter gene expression and activation;
HCV核心蛋白在酵母双杂交系统中的表达及自激活作用检测
3.
Construction and expression of bait vector for NDRG family members and detection of their self-activation in yeast two-hybrid system;
NDRG家族成员酵母双杂交诱饵载体的构建,表达及自激活检测
5)  yeast two hybrid system
酵母双杂交
1.
Design and construction of a random peptide library with yeast two hybrid system;
酵母双杂交随机肽库的设计及构建
2.
AIM: To construct a cycle peptide library composed of 16 random amino acids with yeast two hybrid system.
目的 :构建含 16个氨基酸的酵母双杂交随机环肽库。
3.
Objective To obtain NF κB p65 subunit DNA binding domain cDNA, and measure its autonomous activation in yeast two hybrid system.
目的 获得NF κBp65亚基DNA结合域cDNA及构建酵母双杂交系统中的靶基因 ,并检测靶基因的表达和自身激活活性。
6)  yeast two hybridization
酵母双杂交
1.
Studies on self-activation and self-interaction of circadian clock protein KaiC of Microcystis aeruginosa in yeast two hybridization;
通过PCR扩增了铜绿微囊藻的生物钟主控基因KaiC,并将其分别克隆到酵母双杂交系统的诱饵质粒pGBKT7和猎物质粒pGADT7中,然后将重组质粒pGBKT7-kaiC/pGADT7-kaiC和pGBKT7-kaiC/pGADT7分别共转化酵母菌AH109,经营养缺陷生长和β-半乳糖苷酶印迹检测表明,KaiC蛋白不具有毒性不会影响酵母细胞的生长,也不具有自激活活性,不会激活报告基因的表达,并且KaiC蛋白自身存在较强的相互作用。
补充资料:杂交受精
分子式:
CAS号:

性质:又称异体受精,杂交受精。来自不同个体的配子产生合子的过程。

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