In this paper we reviewed the research tactics for a minimum genome,the methods of reducing genome by markerless deletion,the growth characteristics of the reduced-genome strain and its effect on the plasmid stability,the recombinant protein expression and the metabolism.

Knockout of the Crystalline Inclusion Protein Gene cipA and cipB in Photorhabdus luminescens by RED Homologous Recombination

lincolnensis JLUa2,was generated by gene knockout of lmbU with homologous recombination.

coli W3110 chromosome using the plasmid as follow: Firstly, we knockout the lacI gene using Red-mediated recombineering with overlapping single stranded DNA oligonucleotides.

Construction and application of a vector for gene deletion in gram-negative bacteria

After the construction,the vector was used to carry out gene deletion experiment in the yeeZ gene from Citrobacter freundii.

Breeding,reproducing and identifying for aquaporin-3 gene knock-out mice;

Gene knock-out technology and its application in the research of c-Jun NH2-Terminal Kinase-2

Construction and characterization of a two-component signal transduction system ciaR gene knock-out mutant of Streptococcus suis serotype 2

Effect of p53 gene knockout on cell migration;

Analysis of focal cerebral blood flow,ATP content and protein sythesis of L1 gene knockout mice;

Establishment of a βB2 crystallin gene knockout mice model;