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1)  donor plasmid
供体质粒
1.
The results showed that when taking P50 as a recipient strain,pBac[GOI] as a donor plasmid and pHA4PIG as a helper plasmid,and red fluorescent protein gene,DsRed,driven by an eye-specific promoter,3×p3,as a reporter gene,microinjection into the eggs during 2-3 h after oviposition increased the transformation efficiency.
结果显示:以家蚕品种大造(P50)为供试材料、pBac[GOI]为供体质粒、pHA4PIG为辅助质粒,以眼睛和神经组织特异启动子3×p3启动的红色荧光蛋白基因DsRed为报告基因,在蚕卵产下后2~3h进行注射,综合效果最佳,孵化率和转化率分别达到62。
2)  proton donor
质子供体
1.
The proton donor 4-(p-alkyloxy) benzoic acid were synthesized from 4-hydroxybenzoic acid and 1-bromic alkyl,recrystalized with 95% ethanol,the yield of the 4 intermediates were approximately 20%.
用4-羟基苯甲酸和1-溴代正烷合成了质子供体4-烷氧基苯甲酸,用体积分数为95%的乙醇重结晶,所得4种中间体产率都在20%左右。
2.
proton donor 4-Hydroxide ethylic oxygen benzoic acid were synthesized from 4 -hydroxybenzoic acid , 1 -bromic ethanol and purified with water , yield 82%.
用4-羟基苯甲酸和1-溴代乙醇合成了质子供体4-羟乙氧基苯甲酸,水重结晶,收率82%。
3)  plasmid [英]['plæzmid]  [美]['plæzmɪd]
质粒载体
1.
This study was aimed to investigate the effect of plasmid-mediated RNAi targeting hTERT on blocking hTERT gene and inhibiting telomerase activity in leukemia cell line K562.
本研究探讨质粒载体介导的RNAi靶向hTERT对白血病细胞株K562 hTERT基因封闭、端粒酶活性抑制的作用。
4)  Plasmid vector
质粒载体
1.
Construction and identification of plasmid vector of RNA interference of transforming growth factor-β1 gene
转化生长因子-β1基因RNAi质粒载体的构建与鉴定
2.
A new plasmid vector pMD-Fib-IE-EGFP which contains enhanced green fluorescent protein gene as reportor gene was constructed by subtitute EGFP for GFP in the original plasmid pG350.
以含家蚕丝心蛋白重链基因同源片段的质粒pG35 0为出发质粒 ,插入增强型绿色荧光蛋白 (EGFP)基因 ,构建成以EGFP为报告基因的同源重组质粒载体 pMD Fib IE EGFP ,通过PCR及酶切鉴定表明EGFP以正确的方式插入到原始质粒中 ,通过脂质体介导法转染胃癌细胞能发出很强的绿色荧光 ,表明该质粒能够在真核细胞中表达 ,为进一步建立完善的家蚕转基因系统平台提供基因材
3.
Effective Production and Ex vivo Evaluation of Plasmid Vector Intended for Human Cancer Gene TherapyABSTRCTThe effective production of plasmid vector intended for gene therapy was investigated systematically.
本文较系统地研究了用于肿瘤基因治疗的质粒载体的高效制备工艺。
5)  multimeric plasmid
多体质粒
6)  monomeric plasmid
单体质粒
补充资料:供体
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性质:(一)指在化学反应过程中,能提供某一化学基团的物质(化合物)。例如:L-氨基酸与α-酮酸之间的氨基转移反应,L-氨基酸是氨基的供体。(二)提供基因DNA片段、器官、组织或其他细胞输送给另一个个体的生物。(三)在半导体中,若杂质或缺陷能级上有电子占据时,杂质原子或缺陷是电中性的,这种杂质或缺陷叫做施主,其能级叫做施主能级。施主能级上的电子,如获得一定能量可进入导带;这个能量叫做施主的电离能,也就是施主能级在导带底下方的能量距离。电离能小的施主能级是浅施主能级,电离能大的是深施主能级。磷、砷、锑是锗、硅半导体中的浅能级施主杂质。浅能级施主杂质叫n型杂质。深能级施主杂质可能成为空穴的陷阱或复合中心。

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