Molecular mechanism of insulin resistance induced by free fatty acids in 3T3-L1 adipocytes through targeting nuclear-kB p65;

Expression of adiponutrin mRNA in mature 3T3-L1 adipocytes;

Continuously activated Akt inhibits adiponectin secretion in 3T3-L1 adipocytes;

Obestatin inhibits proliferation and differentiation of 3T3-L1 preadipocytes;

Angiopoietin-like protein 4(ANGPTL4) gene expression during the period of 3T3-L1 preadipocyte differentiation

The differentiation of 3T3-L1 preadipocyte,HL-60,K562 and B16 cells were assessed by microscopy and Oil red O staining,NBT reduction and phagocytosis,haemoglobin(Hb) content and melanin content,respectively.

Establishment of GOD-POD assay in a minimal way and application to glucose metabolism of 3T3-L1 adipocyte and HepG2 cell in vitro;

Akt regulating adiponectin secretion in 3T3-L1 adipocytes;

This paper establishes the insulin resistance model of 3T3-L1 adipocytes.

Effect of emodin on differentiation induction of 3T3-L1 preadipocytes

【Objective】 The aim of this study is to investigate the effect of different concentrations of sporamin on differentiation and proliferation of 3T3-L1 preadipocytes,providing a theoretical basis for the development of prevention and treatment of obesity and anti-diabetes reagent in food industry.

Objective To investigate the effect and underling mechanism of genistein(GEN)on adipogenesis in 3T3-L1 preadipocytes.

Methods After utilizing dexamethasone and insulin to establish insulin-resistant modle by induced 3T3–L1 adipocyte, the levels of tumor necrosis-alpha(TNF–α),interleukin–6(IL–6) and C–reactive protein (CRP) of cell supernatant were detected.