Reverse transcription polymerase chain reaction(RT-PCR),RT-seminested PCR and reverse transcription loop-mediated isothermal amplification(RT-LAMP) assays were compared to detect norovirus in oysters.

Detection of respiratory syncytial virus by semi-nested RT-PCR;

Detection of Tobacco ringspot virus and Tomato ringspot virus by semi-nested RT-PCR;

Detection of Bean pod mottle virus by semi-nested RT-PCR in imported soybean;

Development of nested RT-PCR for detection animal rabies virus

To study the incidence, the types of fusion genes and the clinical significance of rearrangements of mixed lineage leukemia (MLL) gene in acute leukemia (AL), the rearrangements of MLL gene of 60 patients with AL were detected by fluorescence in situ hybridization (FISH) and 6 types of common fusion genes resulting from the rearrangements of MLL gene were detected by nested RT-PCR.

Methods CK19mRNA expression was detected by nested RT-PCR in the peripheral blood from 60 lung cancer patients.

According to the nucleotide sequence of the S1 gene of MDRV registered in GenBank,a set of primers C1、HP11 and HP12 were designed to amplify a specific 300 bp fragment by semi-nested RT-PCR.

A specific semi-nested RT-PCR based on the RNA-dependent RNA polymerase gene in AF469603 of Chinese bee (Apis sinensis) sacbrood virus was established to detect Chinese bee sacbrood virus.

According to the nucleotide sequence of the U25631 gene of Salmonella registered in GeneBank, a set of primers were designed to amplify a specific 581 bp fragment by Semi-nested RT-PCR.

Research on Promoter Region Methylation Status of Tumor Repressor Gene P16 in Human Hepatocellular Carcinoma by Using Methylation Sensitive Restriction Enzyme and Semi-nested PCR Assay;

A comparison of the detection sensitivity of five primer pairs(fOI1/rOI2c,fOI2/r23S1,fA2/rJ5,fP535/rP535,fP400/rP400)for citrus huanglongbing pathogen was carried out using simple PCR,semi-nested PCR and nested PCR.

Semi-nested PCR method was established to detect trace ingredient of transgenic rapeseeds,which was 100 times more sensitive than the regular PCR.

we detected for HPV16DNA in 152 cases of cervical carcinoma and 60 cases of normal cervical epithelia with semi-nested polymerase chain reaction.

To investigate the correlation between HPV16 infection and p53 protein expression in cervical carcinoma among Uigur women in Xinjiang,we detected for HPV16DNA and p53 protein in 80 cases of cervical carcinoma and 34 cases of normal cervical epithelia with semi-nested polymerase chain reaction and immunohistochemistry methods,respectively.