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1)  Elongation technique
延伸技术
2)  extension of technology
技术延伸
1.
This article classified the modes of technical creation into three types by difficulties degree in realization technical creation with greater development,extension of technology and synthesis of technology and compared and analyzed them and then came to an end that the synthesis of technology was a more important way of technical creation in our country today.
把技术创新的途径按实现的难易程度分为重大技术突破的创新、技术延伸的创新和技术综合的创新等 3种方式,并对它们进行了比较和分析,从中得出技术综合是目前我国中小企业技术创新的重要途径,最后论述了企业技术综合的几个主要内容。
3)  splicing by overlap extension
PCR重叠延伸技术
1.
Methods The PCR product of signal and mature peptides of HD-5 were linked by splicing by overlap extension(SOE).
方法通过PCR重叠延伸技术得到HD-5信号肽及成熟肽的嵌合分子,并将其插入真核表达质粒pVITRO3。
2.
The PCR product of signal and mature peptides of GLP2 was linked by SOE (splicing by overlap extension) and the gene, which coded the second amino acid in the N-terminal of GLP2, was mutated from GCT to GGT by PCR.
方法通过RT-PCR方法获得含GLP2编码序列的高血糖素原cDNA片段;通过PCR重叠延伸技术得到GLP2信号肽及成熟肽的嵌合分子,并将编码GLP2成熟肽N-末端第二位丙氨酸的序列(GCT)突变为编码甘氨酸的序列(GGT);将其插入真核表达质粒pVITRO3,转染至肠上皮细胞(Caco-2),观察其对细胞增殖活性的影响。
4)  splicing by overlap extension (SOE)
重叠延伸技术(SOE)
5)  s plicing by overlap extension PCR
重叠延伸PCR技术
6)  staggered extension process
交错延伸技术
补充资料:延伸
1.延长伸展。
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