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1)  P1-2A gene
P1-2A基因
1.
The P1-2A gene and the 3C gene of O/China 99 strain of foot-and-mouth disease virus were amplified by RT-PCR.
采用RT-PCR方法分别扩增口蹄疫病毒O/China99毒株的P1-2A和3C基因,将P1-2A基因连接到pUC119载体,3C基因连接到pMD18-T载体,分别得到重组载体pUC119-P1-2A和pMD18-T-3C;将重组载体pUC119-P1-2A用HindⅢ、BamHⅠ酶切,重组载体pMD18-T-3C用BamHⅠ、NheⅠ酶切;利用酶切所得到的基因片段P1-2A、3C有共同的BamHⅠ酶切位点,实现基因P1-2A、3C的连接,构建重组载体pMD18-T-P1-2A-3C。
2)  P1-2A-3C gene
P1-2A-3C基因
1.
Objective To construct a eukaryotic expression vector for the P1-2A-3C gene of foot and mouth disease virus (FMDV) type Asia 1.
目的构建Asia 1型口蹄疫病毒(FMDV)P1-2A-3C基因真核表达质粒。
2.
Objective To construct the recombinant fowlpox virus (rFPV) for co-expressing the P1-2A-IL-18 gene of foot-and-mouth disease virus (FMDV) type O and P1-2A-3C gene of FMDV type Asia I.
目的构建共表达口蹄疫病毒(FWDV)O型P1-2A-IL-18基因和AsiaI型P1-2A-3C基因的重组鸡痘病毒。
3)  P1/2A/3C gene
P1/2A/3C基因
4)  P1-2A-IL-18 gene
P1-2A-IL-18基因
1.
Objective To construct the recombinant fowlpox virus (rFPV) for co-expressing the P1-2A-IL-18 gene of foot-and-mouth disease virus (FMDV) type O and P1-2A-3C gene of FMDV type Asia I.
目的构建共表达口蹄疫病毒(FWDV)O型P1-2A-IL-18基因和AsiaI型P1-2A-3C基因的重组鸡痘病毒。
5)  gene P1
P1基因
1.
The structural proteins encoded by the gene P1 of foot and mouth disease virus(FMDV)is the foundation for the construction of FMDV capsids which play very important roles for inducing neutralization antibodies and other immune protection.
口蹄疫病毒P1基因编码的结构蛋白是构成口蹄疫病毒衣壳的基础,对诱导动物机体产生中和抗体和其他保护性免疫反应有重要作用。
6)  P1 gene
P1基因
1.
P1 gene was cloned into pGEM-T Easy vector to analyze the nucleotide sequence.
将P1基因、Kozak序列等插入中间表达质粒pB in438,构建重组中间表达载体pB inP1。
2.
To construct the bi-valent genetic engineering vaccine against pseudorabies and foot-and mouth disease,P1 gene of foot-and mouth disease virus was inserted into a PRV universal vector-PgG-uni,and transfer vector PgG-P1 was constructed.
为了构建口蹄疫与伪狂犬病二价基因工程疫苗株 ,将口蹄疫病毒 (FMDV) P1基因插入到伪狂犬病病毒 (PRV)通用载体 p Pg G- uni中 ,得到 PRV转移载体 p Pg G- P1。
3.
FMDV-P1,P1 gene was cloned by RT-PCR and pBI131SP1,pBIP1 and pBIAP1vector was constructed, then the vector was transformed into Agrobacterium tumefaciens (LBA4404 and EHA105).
通过RT-PCR克隆了口蹄疫病毒(FMDV)全长P1基因,然后与植物的表达盒融合构建了重组质粒pBI131SP1、pBIP1和pBIAP1,并将质粒转化到根癌农杆菌LBA4404和EHA105中,获得了植物双元表达载体。
补充资料:[styrene-(2-vinylpyridine)copolymer]
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性质:学名苯乙烯-2-乙烯吡啶共聚物。微黄色粉末或透明小颗粒晶体。无臭,无味。不溶于水,溶于酸、乙醇、丙酮、氯仿。有抗水、防潮性能,适用于多种药片的包衣等。

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