1) chitin affinity chromatography
chitin亲和层析
1.
The fusion protein CBD-intein-Tn Ⅰ was expressed by addition of IPTG and purified by chitin affinity chromatography.
经chitin亲和层析和intein介导的融合蛋白自我裂解,纯化得到重组TnⅠ。
2) Affinity chromatography
亲和层析
1.
Screening the inhibitors for PrA from Ganoderma lucidum by immobilized enzyme affinity chromatography;
固定化亲和层析分离灵芝发酵液蛋白酶A抑制剂
2.
Purification of Glutathione (GSH) by metal-chelated affinity chromatography;
金属螯合亲和层析分离纯化谷胱甘肽(GSH)初探
3.
A new technology of isolation and purification:affinity chromatography;
分离纯化新技术亲和层析
3) Immunoaffinity chromatography
亲和层析
1.
Purification of NGF from Naja naja atra Cantor venom by immunoaffinity chromatography WEI Chuan-bao, HUANG Ya-nan;
舟山眼镜蛇毒神经生长因子的亲和层析分离
2.
Immunoaffinity chromatography was used to purify rhI.
用BmNPV/IGF 1感染家蚕幼虫 ,提取家蚕血淋巴液 ,采用亲和层析法纯化rhIGF 1,ELISA法测定rhIGF 1含量 ,用SDS PAGE和Western blot分析鉴定rhIGF 1纯度及免疫学活性 ,MTT法观察其对MCF 7细胞增殖的影响。
4) Affinity chromatograph
亲和层析
1.
coli BL21and myostatin fusion protein was purified by affinity chromatography.
为研究猪肌生成抑制素(myostatin,MSTN)蛋白的功能及为制备此蛋白的单克隆抗体提供材料,将原核表达菌种MSTN-PGEX-4T-1-BL21进行融合表达,用亲和层析的方法对GST-MSTN和MSTN C-端成熟蛋白进行分离纯化。
2.
The development of affinity chromatography technique is very fast in recent years,remarkable ac-complishments have been achieved in the separation and purification of biomolecules and organisms as well as in studying the interactions between biomolecules.
近几十年来,亲和层析技术发展十分迅速,广泛应用于生物分子(如结合蛋白、酶、抑制剂、抗原、抗体、激素、激素受体、糖蛋白、核酸及多糖类等)及组织(如细胞、细胞器、病毒等)的分离和纯化,是蛋白质组学研究中重要的技术之一。
3.
Based on their specificity, Sepharose CL-6B was adopted in this study as affinity chromatography to separate the S-type lectin in annelid from the earthworm, in which EDTA-MEPBS (2 mmol/L EDTA, 4 mmol/Lβ—mercaptoethanol, 150 mmol/L NaC1, 20 mmol/L phosphate, pH7.
根据其性质，本研究采用惰性分子筛填料Sepharose CL-6B作为亲和层析介质对蚯蚓S型凝集素进行了纯化。
5) Chromatography
[英][,krəumə'tɔgrəfi] [美][,kromə'tɑgrəfɪ]
亲和层析
1.
Bind Resin Chromatography under denaturing condition and dialyzed for renaturation, and then were analyzed with SDS-PAGE, West.
目的探讨通过金属螯合亲和层析的方法获得高纯度的人内皮细胞高表达脂多糖相关因子1(EOLA1)蛋白的可行性。
2.
The fusion protein was purified by chromatography Glutathione Sepharose 4B and digested by 3C protease.
用glutathioneSepharose 4B亲和层析柱纯化表达产物后 ,以重组 3C蛋白酶对融合蛋白进行解离 ,SDS PAGE对表达及纯化产物进行鉴定。
补充资料：Chitin
壳聚糖是甲壳素的脱乙酰化产物，又称可溶甲壳素、壳多糖、甲壳胺，是一种天然生物高分子聚合物，白色结品性扮末。有很强的吸湿性，仅次于甘油，高于聚乙二醇、山梨醇。在吸湿过程中，分子中的羟基、胺基等极性基团与水分子作用而水合，分子链逐渐膨胀，随着pH值的变化，分子够从球状胶束变成线状。具有很好成膜性、透气性和生物相容性。无毒，且可生物降解。
用作水溶性胶粘剂的增稠剂。
用作水溶性胶粘剂的增稠剂。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条