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1)  antibody against AFB1
抗黄曲霉毒素B1 scFv菌株
1.
The competitive ELISA confirmed that three strains,3C3,3B3 and 4A2,could secret the soluble scFv antibody against AFB1.
进一步以噬菌体感染大肠杆菌HB2151,在细菌细胞周质中可溶性大量表达,最终经竞争ELISA分析表明获得了3株可以稳定分泌抗黄曲霉毒素B1 scFv菌株,分别命名为3C3、3B3和4A2。
2)  Aflatoxin-producing Strains
产黄曲霉毒素菌株
3)  aflatoxin B1
黄曲霉毒素B1
1.
Screening and Identification of Aflatoxin B1 Degradation Strains;
黄曲霉毒素B1降解菌株的筛选及鉴定
2.
Preparation of Monoclonal Antibody and Single-chain Antibody Fragment to the Hapten Aflatoxin B1;
黄曲霉毒素B1单克隆抗体和单链抗体的制备
3.
Construction of an Anti-aflatoxin B1 scFv Library and Screening for Specific Antibodies Against AFB1
抗黄曲霉毒素B1单链抗体(scFv)库的建立和筛选
4)  Aflatoxin B_1
黄曲霉毒素B1
1.
The high-performance liquid chromatographic(HPLC) method with the fluorescence detector(FLD) to analyse aflatoxin B_1 in cereals of extracting samples into chloroform,cleaning-up on a LC-Si SPE tube and reacting with trifluoroacetic acid to form a ramification has been established.
建立了用三氯甲烷提取、硅胶小柱净化、三氟乙酸衍生,再以荧光检测器来测定粮谷中黄曲霉毒素B1的高效液相色谱方法。
2.
AIM To study if there are different effects of 9 enantiomers of clausenamides on aflatoxin B_1(AFB_1)-induced unscheduled DNA synthesis(UDS) injury and glutamic-pyruvic transferase(GPT) release of isolated rat hepatocytes in vitro.
目的研究9种光学活性黄皮酰胺类化合物体外对黄曲霉毒素B1(AFB1)引起大鼠肝细胞非程序性DNA合成(UDS)损伤和谷丙转氨酶(GPT)释放的保护作用有无差异。
3.
Preparation of Polyclonal Anti-Idiotypic Antibody Against Anti-Aflatoxin B_1 Monoclonal Antibody
目的研制黄曲霉毒素B1标准品的替代品并应用于酶联免疫吸附试验(ELISA)。
5)  AFB1
黄曲霉毒素B1
1.
The results showed that the aflatoxin B1(AFB1) and benzo(a) pyrene contents of the sample after degumming,deacidification,bleaching and deodorization were 21 μg/kg and 11 μg/kg,which respectively exceed 5% and 10% of the corresponding indexes in the national hygienic standard for edible vegetable oil.
结果表明,经脱胶、脱酸、脱色、脱臭后的样品黄曲霉毒素B1及苯并(a)芘高达21μg/kg和11μg/kg,分别超出国家规定食用植物油卫生标准的5%和10%,且其电导率及极性成分也远高出市售食用植物油。
2.
Backgrounds and Aims:The morbility of hepatocellular carcinoma(HCC) is highly associated with aflatoxin B1(AFB1)contaminating in food.
背景与目的:肝细胞癌(Hepatocellular carcinoma,HCC)的发病与食物被黄曲霉毒素B1(aflatoxin B1,AFB1)污染密切相关。
3.
In order to study absorption characteristics and mechanisms of Lactobacillus plantarum to aflatoxin B1 (AFB1), 14 strains with absorption capability to AFB1 were screened in vitro.
采用体外筛选法从14株乳酸杆菌中筛选能吸附黄曲霉毒素B1(AFB1)的菌株,并探讨其吸附特性和机理。
6)  aflatoxin B 1
黄曲霉毒素B1
1.
After being extracted with CH 3OH H 2O,the sample extract was filtered, diluted with water and applied to affinity column containing monoclonal specific antibody for aflatoxin B 1.
试样经过甲醇 水提取 ,提取液经过滤、水稀释、用键合有黄曲霉毒素B1特殊抗体的免疫亲和柱净化 ,通过高效液相色谱仪测定黄曲霉毒素B1。
2.
After 32 days,rats of group Ⅱ,Ⅲ and Ⅳ were treated with the program“short-term animal hepatocarcinoma model induced by aflatoxin B 1(AFB 1)”.
各组饲喂基础料, 至体重增长到150~200g(第 32天) 时, 除组Ⅰ外, 其余 3组大鼠实施“黄曲霉毒素B1 (aflatoxinB1, AFB1 ) 致肝癌短期实验模型程序”, 并测定肝、肾组织和血中硒含量及谷胱甘肽过氧化物酶 (GSH Px)、超氧化物歧化酶 (SOD) 和γ GT酶活性、丙二醛 (MDA) 和一氧化氮 (NO) 含量。
3.
Objective Assay the Aflatoxin B 1 in the foodstuff and feedstuff.
目的 检测食品、饲料中黄曲霉毒素B1 。
补充资料:黄曲霉
分子式:
CAS号:

性质:谷类及其他农作物常见的霉变菌。最适生长温度为37℃左右,生长温度范围为6~46℃。由黄曲霉和寄生曲霉(Asp. parasitieus)产生的一类低分子化合物(分子量350左右)——黄曲霉毒素。污染粮食、油料作物的种子,其中以花生、玉米最易被污染。黄曲霉毒素的种类很多,其中以黄曲霉毒素B1毒性最强。结晶黄曲霉毒素B1极为稳定,高温(200℃)、紫外线照射都不受破坏。对动物、植物和微生物具有很强的毒性,致癌力最强,还能引起突变和导致畸形。与人的肝癌有密切关系,也能引起人的急性中毒死亡。在中性、酸性溶液中很稳定。在强碱溶液中能有效降解。

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