1) real-time fluorescence quantitative polymerase chain reaction
实时荧光定量聚合酶链反应
1.
Fetal DNA in maternal plasma was measured using real-time fluorescence quantitative polymerase chain reaction(FQ-PCR)for the SRY gene on the Y chromosome.
方法:从50例妊娠7、8、9周的孕妇外周血血浆中提取胎儿游离DNA,用实时荧光定量聚合酶链反应(FQ-PCR)技术检测其中的Y性别决定区(SRY)基因。
2.
Fetal DNA in maternal plasma was measured using real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) for the SRY gene on the Y chromosome.
方法:从孕早期50 名孕妇外周血血浆中提取胎儿DNA,用实时荧光定量聚合酶链反应(fluorescence quantitative polymerase chain reaction, FQ-PCR)技术检测其中的Y 性别决定区(sex-determining region Y, SRY)基因。
2) real-time polymerase chain reaction
实时荧光定量聚合酶链反应
1.
Methods DNASE1 mRNA expression was detected by real-time polymerase chain reaction and its alternatively spliced transcripts were performed by capillary electrophoresis.
方法 以实时荧光定量聚合酶链反应 (real- time PCR)方法检测 DNASE1的 m RNA表达水平 ,以毛细管电泳技术分析 m RNA编码区替代剪接体 ,结合单核苷酸多态性 (single nucleotide polymorphisms,SNPs)单倍型了解基因结构对表达的影响。
3) real-time fluorescence quantitative PCR
实时荧光定量聚合酶链反应
1.
Methods The Expreesion of Myocardial Sodium-Hydrogen Exchanger 1 mRNA in 35 heart failure patients was detected by using real-time fluorescence quantitative PCR (FQ-PCR).
方法采用实时荧光定量聚合酶链反应(FQ-PCR)检测35例心力衰竭患者心肌组织NHE1 mRNA的表达水平。
4) real-time quantitative polymerase chain reaction
实时荧光定量聚合酶链反应
1.
Application of real-time quantitative polymerase chain reaction for research about periodontology;
实时荧光定量聚合酶链反应技术在牙周相关研究中的应用
6) real-time reverse transcription polymerase chain reaction
实时荧光定量反转录聚合酶链反应
1.
Methods The expression of PBMC IP-10 mRNA in 42 children with KD and 40 healthy children were assayed by real-time reverse transcription polymerase chain reaction(real-time RT-PCR).
方法实时荧光定量反转录聚合酶链反应(real-time RT-PCR)检测42例KD及40例健康儿童PBMC中IP-10 mRNA表达。
补充资料:聚合酶链反应
聚合酶链反应
polymerase chain reaction,PCR
是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA)片断大量增扩,达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体,因其假阳性率较高,故应谨慎使用。
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参考词条