1) Phage antibody library
噬菌体抗体库
1.
Construction of phage antibody library using sensitized in vitro B-lymphocytes of liver cancer patients;
用体外致敏联合EB病毒转化技术构建抗肝癌的人源Fab噬菌体抗体库
2.
Construction and preliminary identification of humanized Fab fragment phage antibody library against Schistosoma japonica;
人源性抗日本血吸虫噬菌体抗体库的构建及初步鉴定
3.
Construction of single-chain phage antibody library of cancer-bearing EL-4 mice;
EL-4荷瘤鼠单链噬菌体抗体库的构建
2) phage display antibody library
噬菌体抗体库
1.
Construction and screening of phage display antibody library directed against the extracellular domain of human integrin β_3;
抗整合素β_3胞外区噬菌体抗体库的构建及筛选
2.
Construction of humanized phage display antibody library for human cytomegalovirus;
抗HCMV人源化噬菌体抗体库的构建
3.
Phage display antibody library technology, which has been widely used in the diagnosis and treatment of disease, and functional protein expression, was a high-performance, time saving, and effective technique for producing specific antibodies.
噬菌体抗体库技术是继单克隆抗体制备技术后具有换代地位的抗体制备技术，在功能蛋白的表达、疾病诊断和治疗等领域得到了广泛研究，用在农兽药等小分子抗体的制备上也获得了亲和力较高的抗体。
3) Phage display library
噬菌体抗体库
1.
The functional binding antibodies to novel purinoceptor selected from phage display library;
从噬菌体抗体库中筛选血管内皮细胞新型嘌呤受体功能抗体的研究
2.
Objective To construct Fab phage display library against human B-lymphoma.
目的构建B细胞淋巴瘤Fab噬菌体抗体库。
3.
Objective The purpose of this study was to obtain the high-affinity single-chain Fv antibody against platelet membrane glycoprotein(GP) Ⅱb/Ⅲa from anti-activity platelet phage display library.
方法 从活化血小板免疫的小鼠脾淋巴细胞中提取总RNA ,反转录成cDNA后 ,用抗体可变区混合引物扩增全套轻、重链可变区基因 ,经重叠延伸反应 ,装配成单链抗体 (ScFv)基因 ,将其克隆到噬菌体载体pHEN1中 ,构建单链抗体噬菌体抗体库。
4) T7 phage display library
T7噬菌体抗体库
1.
A T7 phage display library of human single-chain fragment of variable region (scFv) was constructed to screen antibodies against nucleocapsid protein (NP) of Hantaan virus.
结果表明,研究成功构建了人源抗NP蛋白T7噬菌体抗体库。
5) Fab phage antibody library
Fab噬菌体抗体库
1.
Objective To construct a human specific Fab phage antibody library for screening Fab against HBsAg.
目的 构建人源特异性抗乙型肝炎病毒表面抗原Fab噬菌体抗体库。
补充资料：噬菌体展示库
噬菌体展示库(phage display library)的基本原理是将外源基因同丝状噬菌体fd或mb的外壳蛋白p8基因或p3基因融合后导入噬菌体基因组，表达产生的外源肽与外壳蛋白p8或p3形成融合蛋白，展示在噬菌体的表面。导入了各种各样外源基因的一群噬菌体，就构成一个展示各种各样外源肽的噬菌体展示库。当用一个蛋白质去筛查一个噬菌体展示库时，就会选择性地同与其有相互作用的某个外源肽相结合，从而分离出展示库里的某个特定的噬菌体，研究该噬菌体所含外源基因的生物学功能。 基于同一原理的另一种实验系统是将编码细菌表面的菌毛蛋白的基因，同外源基因融合，转入细菌细胞后表达，在细菌表面的菌毛中出现融合蛋白。当遇到能同外源基因的蛋白质产物相互作用的蛋白质时就能选择性地与融合蛋白相结合。这样，也就起到展示库的作用，可以筛选有相互作用的蛋白质。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条