1) Whole genome amplification
全基因组扩增
1.
Preimplantation genetic diagnosis for β-thalassemia using whole genome amplification;
应用全基因组扩增技术对β-地中海贫血进行胚胎植入前遗传学诊断
2.
Methods Whole genome amplification (WGA) was performed for trace DNA using modified IPEP, and the amplified products were quantitated by real-time quantitative PCR as well as genotyped by AmpFLSTR~ Indentifiler~ kit.
方法用改良IPEP法对痕量样品DNA进行全基因组扩增(WGA),扩增产物用实时荧光定量PCR技术定量、用AmpFLSTR~ Indentifiler~试剂盒作基因型检测。
3.
Objective To establish a method for whole genome amplification (WGA) based on (multiple displacement amplification MDA), and achieve DNA analysis of the human genome from low copy number (LCN).
目的建立基于多重置换扩增(MDA)技术的全基因组扩增(WGA)方法,实现对低拷贝数(LCN)DNA样品进行分析。
2) whole genome amplification of a single cell
单个细胞全基因组扩增
3) genome amplification
基因组扩增
1.
The genome of sindbis virus was amplified by random PCR and MDA strategies respectively, and the products were used as template to amplify specific gene in it to test the quality of the two genome amplification method; then the genome amplification products of sindbis virus were labeled with fluorescence dye and detected with microarray.
为了摸索均衡的病毒基因组扩增方法,建立高通量的病毒检测基因芯片技术平台,本研究以甲病毒属的辛德比斯病毒作为检测模型,分别以随机PCR扩增法和MDA(multipledisplacementamplification)扩增法扩增病毒基因组,并以两种扩增产物作为模板,扩增辛德比斯病毒的特异基因片段以初步验证基因组扩增的均衡性;然后将两种基因组扩增产物标记荧光染料后与基因芯片进行杂交;结果表明从两种基因组扩增产物中正确扩增出了辛德比斯的特定基因片段,作为探针可与基因芯片上的靶标基因特异性结合;基因组扩增产物与基因芯片进行杂交,可成功检测到甲病毒属的特异性信号,充分说明随机PCR扩增法和MDA扩增法扩增病毒基因组可用于病毒性病原体的基因芯片检测。
5) high fidelity genome-wide mRNA amplification technique
高可信度全基因组mRNA扩增技术
1.
Establishment of laser captured microdissection-high fidelity genome-wide mRNA amplification technique;
激光捕获显微分离-高可信度全基因组mRNA扩增技术的建立及其在食管癌细胞基因检测中的应用
6) whole genome duplication
全基因组倍增
1.
In this review,we focus on four recent advances in rice genome analysis: an whole genome duplication which is believed to occur in rice genome; evolution of rice genome size; the indica-japonic divergence that was likely to occur later than the previous estimate of 1 million years ago; hypothesis for GC compositional gradients in rice genes.
内容主要包括:水稻基因组被证实曾发生过全基因组倍增;水稻基因组大小变化的趋势;水稻籼粳亚种的分化时间比以前估计的100万年前要迟得多;对水稻基因产生GC含量负梯度现象的解释。
补充资料：全增嘏
全增嘏
浙江绍兴人。民盟成员。1925年获美国丹佛大学学士学位，1926年获哈佛大学研究学院硕士学位。1928年回上海，历任大同大学、中国公学、光华大学、暨南大学、大夏大学教师，中国评论周报助理编辑，《天下月刊》(英文)编辑，复旦大学外语系教授、主任、图书馆长。1931年开始发表作品。
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