1) electrophoretic m obility shift assay
凝胶迁徙率泳动分析
2) electrophoretic mobility shift assay(EMSA)
凝胶电泳迁移率变动分析
1.
Furthermore, electrophoretic mobility shift assay(EMSA) showed that DIG-ddUTP labeled DNA sequences containing ezrin key elements could bind nuclear extracts from lung cancer cells to form DNA-protein complex, and the bindings to Sp1 and AP-1 sites by rhSp1 and rhAP-1, respectively, were specific.
其次,利用凝胶电泳迁移率变动分析证明,肺癌细胞核蛋白提取物能够与ezrin基因含有关键顺式作用元件的DNA序列结合,形成DNA-核蛋白复合物,而且Sp1结合位点和AP-1结合位点与重组蛋白rhSp1和rhAP-1的结合具有位点特异性。
3) Gel Mobility Shift Assay
凝胶迁移率变动分析
1.
In order to investigate the regulatory mechanism of LIP genes at transcriptional level, 6 DNA fragments subcloned from 5′ upstream sequences of LIP genes ( GLG 3 and GLG 6) were obtained, then gel mobility shift assay was carried out to screen DNA fragment(s) with ability to be bound specifically by proteins isolated from P.
为研究ＬＩＰ基因的转录调控机理， 对ＬＩＰ基因（ ＧＬＧ３ 和ＧＬＧ６） 的５′端上游序列进行亚克隆， 获得６ 个亚克隆ＤＮＡ 片段， 然后应用凝胶迁移率变动分析技术筛选能与菌体蛋白质专一性结合的ＤＮＡ片段。
4) assay
[英][ə'seɪ] [美][ə'se]
凝胶迁移率变动分析，凝胶移位分析
5) Gel mobility shift asssy
凝胶迁移率分析
6) electrophoretic mobility shift assay
电泳迁移率变动分析
1.
Objective:To establish a fast and simple method of electrophoretic mobility shift assay(EMSA)for the identification of DNA-binding protein nuclear factor-kappa B(NF-κB).
目的:建立一种快速、简化的电泳迁移率变动分析(EMSA)方法检测DNA结合蛋白核因子-κB(NF-κB)。
2.
The combination of Nanog and DNA fragments was proved by electrophoretic mobility shift assay(EMSA).
结果:我们发现fzr为Nanog调控的目的基因,并通过电泳迁移率变动分析证明了二者的结合。
补充资料：Sds-聚丙烯酰胺凝胶电泳凝胶电泳
sds-聚丙烯酰胺凝胶电泳（sds-page，sodium dodecyl sulfate-polyacrylamide gel electrohoresis）
在有去污剂十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳。sds-page只是按照分子大小分离的，而不是根据分子所带的电荷和大小分离的。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条