1) electro-phoretic mobility shift assay
电泳迁移率分析
2) electrophoretic mobility shift assay
电泳迁移率变动分析
1.
Objective:To establish a fast and simple method of electrophoretic mobility shift assay(EMSA)for the identification of DNA-binding protein nuclear factor-kappa B(NF-κB).
目的:建立一种快速、简化的电泳迁移率变动分析(EMSA)方法检测DNA结合蛋白核因子-κB(NF-κB)。
2.
The combination of Nanog and DNA fragments was proved by electrophoretic mobility shift assay(EMSA).
结果:我们发现fzr为Nanog调控的目的基因,并通过电泳迁移率变动分析证明了二者的结合。
3) Electrophoretic mobility shift assay
电泳迁移率改变分析
1.
Methods The activation of NF-κB in intestinal and lung tissue of rabbit intestinal I/R model was detected by electrophoretic mobility shift assay (EMSA).
方法建立小肠缺血再灌注家兔模型,采用电泳迁移率改变分析方法(electrophoreticmobilityshift assay,EMSA),分析服用活血承气合剂后不同时段小肠缺血再灌注家兔小肠以及肺组织NFκB活性的变化。
2.
Methods The important cis-acting elements of NHE 1 gene and the transcription factors binding to them in lung tumor cells were examined with electrophoretic mobility shift assay (EMSA) and their mutual actions were studied.
方法 采用电泳迁移率改变分析法 (EMSA)检测人肺癌细胞株核蛋白中C EBP和HMG样蛋白的表达及其水平。
4) EMSA
电泳迁移率变动分析(EMSA)
5) electrophoretic mobility shift assay
电泳迁移率
1.
Objective To establish a new non-radioactive method for electrophoretic mobility shift assay (EMSA) to investigate the binding between glucocorticoid induced leucine zipper (GILZ) and peroxisome proliferator-activated receptor-gamma 2 (PPARγ2) promoter oligonucleotides.
目的通过红外荧光技术研究糖皮质激素诱导亮氨酸拉链蛋白(GILZ)与过氧化物酶体增殖物活化受体γ2(PPARγ2)启动子寡核苷酸的结合,了解一种非放射性研究电泳迁移率变动分析的新方法。
2.
Methods The TTF-1 DNA binding activity was detected in lung cancer tissue by Electrophoretic mobility shift assay(EMSA),autoradiography and photo densitometry.
方法采用电泳迁移率(Electrophoretic mobility shift assay,EMSA)同位素放射自显影,光密度分析的方法分别检测有随访资料的96例肺癌组织中TTF-1的DNA结合活性。
6) electrophoretic mobility shift assay(EMSA)
凝胶电泳迁移率变动分析
1.
Furthermore, electrophoretic mobility shift assay(EMSA) showed that DIG-ddUTP labeled DNA sequences containing ezrin key elements could bind nuclear extracts from lung cancer cells to form DNA-protein complex, and the bindings to Sp1 and AP-1 sites by rhSp1 and rhAP-1, respectively, were specific.
其次,利用凝胶电泳迁移率变动分析证明,肺癌细胞核蛋白提取物能够与ezrin基因含有关键顺式作用元件的DNA序列结合,形成DNA-核蛋白复合物,而且Sp1结合位点和AP-1结合位点与重组蛋白rhSp1和rhAP-1的结合具有位点特异性。
补充资料：Sds-聚丙烯酰胺凝胶电泳凝胶电泳
sds-聚丙烯酰胺凝胶电泳（sds-page，sodium dodecyl sulfate-polyacrylamide gel electrohoresis）
在有去污剂十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳。sds-page只是按照分子大小分离的，而不是根据分子所带的电荷和大小分离的。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条