In this study, the DNA sequence and corresponding amino acid sequence of exogenous CP4-EPSPS gene from GM Roundup-Ready rapeseed and soyabean were compared, and two pairs of primers were designed and the synthetic CP4-EPSPS gene and Agrobacterium CP4-EPSPS gene were detected respec.

The genetically modified Roundup-ready canola was quantitatively detected by ABI Prism 7700 sequence detector with the PCR primers and TaqMan fluorescence probes designed according to the sequences of endogenous PEP gene and exogenous GOX gene.

The genotypes 7039 and 7040 were used to select regenerated plants which showed glyphosate resistance and the other two genotypes were used for haloxyfop resistance selection.

In this study, Glyphosate resistance gene has been carried out genetic analysis and marked, losated by the use of SSR molecular markers ,with the Northeast Agricultural University glyphosate resistant strains 89-09 and 89-05-3, and susceptible strains 89-13 and 89-21 for the test materials, through the F2 groups of the two sets of hybrid combinations 89-05-3/89-13 and 89-09 / 89-21.

Its glyphosate resistance, growth optimal pH and antibiotic sensitivity were detected.