The present paper covers the Hopf points bifurcating from double fold points, and gives some computable nondegeneracy conditions and stability conditions of these Hopf points.

The refolding reaction of human group Ⅹ phospholipase A2 in vitro was depended on temperature, pH and protein concentration.

After solubilization, active proteins can be generated through an appropriate refolding process.

The unfolding and refolding of Bacillus amyloliquefaciensα-amylase induced with urea or guanidine hydrochloride were studied by using its intrinsic fluorescence emission spectroscopy,fluorescence phase diagram,fluorescence probe,fluorescence quenching, Native-PAGE,SDS-PAGE and Size exclusion chromatography.

The research on high building demolish by blasting of bidirectional synchronization collapse,on the same floor on the same structure at the same time,in order to demolish the high building with shearing force wall which have inner routway and prefabricate floor in complicated surroundings.