In vitro growth pattern of ADSCs and effects of rhBMP-2 transfection on growth and proliferation of ADSCs;

ADSCs seems to be the ideal seed cell in bone tissue engineering because they can be obtained easily,have the ability of ossification and better amplification in vitro.

ChapterⅠ:Construction and transfection of eukaryotic expression vector for rat transforming growth factor-β_1 Objective To master the method of construction and transfection of eukaryotic expression vector for rat transforming growth factor-β_1 and to study the possibility of gene transfection to adipose-derived stem cells (ADSCs)with this vector.

Cultivation and Differentiation into Neurons of ADSCs from Rat as well as the Expression of BDNF Gene and Receptor of trkB;

Part 1:Study of Adipose tissue-derived stem cells of SD rats by serial explant after enzymatic predigestion Objective:To harvest Adipose tissue-derived stem cells(ADSCs) of SD rats by serial explant after enzymatic predigestion in vitro,in order to obtain a new cultivation method of ADSCs and compare the results with that from Zuk s method.

[Objective]To evaluate the effect of not induced adipose derived stem cells(ADSCs)with calcium alginate healing full-thickness cartilage defects of rabbit knee after the model was made in center of femoral facies patellaris artificially,in order to find a simple and working way of articular cartilage defect restoration.

Objective To observe the expression of rhBMP-2 & VEGF gene that were transfected into adipose-derived stem cells(ADSCs)in vitro,and application of the tissue-engineered bone with the transfected compound to repair the bone defect of ulna of Guizhou minipigs.

Objectives To construct the recombinant adenovirus that express core banding factor 1(Cbfa1) and to isolate adipose-derived stem cells(ADSCs) for searching therapy genes and carrier cells for genes enhancing bone tissue engineering.