Research on Promoter Region Methylation Status of Tumor Repressor Gene P16 in Human Hepatocellular Carcinoma by Using Methylation Sensitive Restriction Enzyme and Semi-nested PCR Assay;

A comparison of the detection sensitivity of five primer pairs(fOI1/rOI2c,fOI2/r23S1,fA2/rJ5,fP535/rP535,fP400/rP400)for citrus huanglongbing pathogen was carried out using simple PCR,semi-nested PCR and nested PCR.

Semi-nested PCR method was established to detect trace ingredient of transgenic rapeseeds,which was 100 times more sensitive than the regular PCR.

we detected for HPV16DNA in 152 cases of cervical carcinoma and 60 cases of normal cervical epithelia with semi-nested polymerase chain reaction.

To investigate the correlation between HPV16 infection and p53 protein expression in cervical carcinoma among Uigur women in Xinjiang,we detected for HPV16DNA and p53 protein in 80 cases of cervical carcinoma and 34 cases of normal cervical epithelia with semi-nested polymerase chain reaction and immunohistochemistry methods,respectively.

Detection of respiratory syncytial virus by semi-nested RT-PCR;

Detection of Tobacco ringspot virus and Tomato ringspot virus by semi-nested RT-PCR;

Detection of Bean pod mottle virus by semi-nested RT-PCR in imported soybean;

Reverse transcription polymerase chain reaction(RT-PCR),RT-seminested PCR and reverse transcription loop-mediated isothermal amplification(RT-LAMP) assays were compared to detect norovirus in oysters.