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1)  nested PCR-SSP typing
巢式PCR-SSP
2)  nested PCR
巢式PCR
1.
Amplify of fetal SRY gene in maternal plasma by using nested PCR;
应用巢式PCR扩增孕妇血浆中胎儿SRY基因
2.
Cloning of N-terminal deleted IκBα gene by nested PCR;
应用巢式PCR法克隆N端部分缺失的IκBα基因
3.
Establishment of a nested PCR assay for detection of Mycoplasma ovis;
羊附红细胞体巢式PCR检测方法的建立及临床应用
3)  Nested RT-PCR
巢式RT-PCR
1.
Development of nested RT-PCR for detection animal rabies virus
动物狂犬病病毒巢式RT-PCR检测方法的建立
2.
To study the incidence, the types of fusion genes and the clinical significance of rearrangements of mixed lineage leukemia (MLL) gene in acute leukemia (AL), the rearrangements of MLL gene of 60 patients with AL were detected by fluorescence in situ hybridization (FISH) and 6 types of common fusion genes resulting from the rearrangements of MLL gene were detected by nested RT-PCR.
为了研究混合系白血病(MLL)基因重排在急性白血病(AL)中的发生率、融合基因类型及其临床意义,用荧光原位杂交技术检测60例急性白血病(AL)患者MLL基因重排,对于MLL基因重排阳性的患者,用巢式RT-PCR方法检测MLL基因重排形成的6种常见融合基因类型。
3.
Methods CK19mRNA expression was detected by nested RT-PCR in the peripheral blood from 60 lung cancer patients.
方法以CK-19为标记,采用巢式RT-PCR和半定量方法对肺癌患者外周血中微转移灶的检测,比较治疗前后外周血中癌细胞数的相对变化。
4)  Nest PCR
巢式PCR
1.
A new method for identifing the change of single nucleotide by created restriction site PCR and Nest PCR;
联合应用巢式PCR和创造酶切位点法检测单核苷酸改变
2.
Utilization of Nest PCR in Intestinal Infection Caused by F subgenus of Enteric Adenovirus
巢式PCR在腺病毒F亚属引起的致腹泻肠道感染检测中的应用
3.
Methods The expressive levels of α1 integrin in specimens were determined by FQ-PCR technique and nest PCR.
方法应用荧光定量PCR和巢式PCR技术检测10例增生性瘢痕组织中整合素α1的表达,并以10例正常皮肤组织作为对照。
5)  nested-PCR
巢式PCR
1.
Nested-PCR detection of human cytomegalovirus, Epstein-Barr virus, herpes simplex virus type 1 in the subgingival plaques and association with chronic periodontitis;
巢式PCR检测龈下菌斑中HCMV、EBV、HSV-1与慢性牙周炎活动性的关系
2.
Detection of Fusarium oxysporum f.sp.cucumarinum by PCR-RFLP and Nested-PCR;
应用PCR-RFLP和巢式PCR检测黄瓜尖镰孢菌
3.
Detection of Fusarium oxysporum f.sp.cucumbrum by nested-PCR;
应用巢式PCR检测黄瓜尖镰孢菌(Fusarium oxysporum f.sp.cucumbrum)
6)  nested polymerase chain reaction
巢式PCR
1.
Sexing of early sheep embryos by using nested polymerase chain reaction;
绵羊早期胚胎性别鉴定巢式PCR体系的建立及其灵敏度研究
2.
Methods Tissue blocks of lymph nodes from 32 true HNL patients and 13 normal subjects were confirmed for parvovirus B19 infection by nested polymerase chain reaction and immunohistochemical techniques in a double blind manner.
方法采用双盲法在32例真性HNL患者及13例正常对照淋巴结活检组织中用巢式PCR和免疫组化染色检测B19病毒基因组DNA和病毒蛋白。
3.
Methods The specific HHV-7 sequences were examined with a nested polymerase chain reaction(PCR) procedure in the lesional skin, plasma, peripheral blood mononuclear cells (PBMC), saliva, and urine samples of 22 cases of acute pityriasis rosea patients as well as in the saliva, plasma and PBMC of 14 convalescent pityriasis rosea patients.
方法采用巢式PCR检测了22例PR急性期患者的血浆、外周血单核细胞(PBMC)、皮损、唾液、尿液,14例恢复期患者的唾液、血浆、外周血单核细胞,14例正常人的唾液、血浆、外周血单核细胞中的HHV-7特异性序列。
补充资料:SSP
分子式:CAH4O2P8.H2O
分子量:
CAS号:

性状:无色或浅灰色颗粒状。
溶解情况:大部分易溶于水,少部分不溶于水而溶于2%柠檬酸溶液中。
用途:一种肥料。可作追肥、种肥和基肥等。适用于各种作物,较适用于中性或碱性土壤。
制备或来源:由硫酸处理磷矿石而得。
备注:主要有效成分是磷酸二氢钙、磷酸氢钙和少量磷酸,还含有无水硫酸钙。含有效无氧化二磷14~20%。加热时性能不稳定。 过磷酸钙是一种速效肥。用少量(每亩10~20kg)磷肥作种肥,有利于作物幼苗吸收磷素,减少土壤对磷的化学固定,是一种经济用肥的好办法。用作作物开花前后的根外追肥效果也很好,喷施浓度一般为1%~3%。为了提高过磷酸钙的肥效,应掌握基肥深施、集中施用的原则,此外,把磷肥四于前茬豆科作物(如绿肥)上,发挥“以磷增氮”的作用,磷肥与有机肥料混合施用,以及磷肥与氮肥配合施用等做法,均能提高磷肥的利用率,增进效果,
包装及贮运:用编织袋内衬塑料薄膜,衬袋材料可用高密度聚氯乙烯薄膜、改性聚乙烯薄膜或低密度聚乙烯薄膜。贮运中防潮,以免结块溶失,要避免日晒雨淋,减少养分损失。车船上要铺垫耐腐蚀的垫板和蓬布。
类别:磷肥


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