1) splicing-by-overlap extension
基因重叠延伸
2) gene splicing by overlapping extensionPCR
基因重叠延伸拼接PCR
3) overlap extension
重叠延伸
1.
The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.
建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。
4) Overlap-extension
重叠延伸
1.
Amplification of DNA sequence Encoding N-terminal Fragment of Osteoprotegerin Using Overlap-extension PCR;
重叠延伸PCR法在合成人骨保护素N端编码序列中的应用
5) signal peptide
重叠延伸
1.
CAD, then using SOE(splicing by overlap extension) technique to fuse CD137 signal peptide gene into LIGHT extramembrane domain encoding sequence, inserting this recombinated LIGHT cDNA with human IgG1 Fc cDNA into the eukaryotic expression plasmid pcDNA3, .
测序证实后 ,继续用PCR扩增其膜外区cDNA ,用重叠延伸技术(SOE)引入CD137信号肽基因。
6) Overlap extension PCR
重叠延伸PCR
1.
1 as the template,the PSD-95 mutant genes were amplified by overlap extension PCR,and then were cloned into the plasmids of PSD-95-pcDNA3.
1为模板,采用重叠延伸PCR法获得突变型PSD-95的局部或全长cDNA片段,并克隆至PSD-95-pcDNA3。
2.
According to the amino acids sequence of OC-IΔD86 gene and Escherichia coli codon usage, we synthesized this gene by overlap extension PCR method with 7 oligonucleotides DNA fragments.
根据OC-IΔD86基因序列,设计合成了7条寡核苷酸片段,通过重叠延伸PCR技术合成了OC-IΔD86基因,利用设计好的BamH I/Xho I酶切位点将OC-IΔD86基因克隆到原核表达载体pet21b中,在1mmol/L的IPTG诱导后5h,OC-I?D86融合基因在大肠杆菌中得到表达,表达产物处于可溶状态,其表达量占总蛋白的11。
3.
How to create two separate site-specific mutagenesis in a DNA fragment using overlap extension PCR was explored.
探讨如何利用重叠延伸PCR对同一靶DNA片段中的两个不同位点实施联合突变。
补充资料：重叠基因
分子式：
CAS号：
性质：这是一种现象，同一部分的DNA序列，具有合成两种不同蛋白质的信息。只要读码移动一个或两个核苷酸，两种不同蛋白质的翻译便可完成。
CAS号：
性质：这是一种现象，同一部分的DNA序列，具有合成两种不同蛋白质的信息。只要读码移动一个或两个核苷酸，两种不同蛋白质的翻译便可完成。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条