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1)  absolute quantitative PCR
绝对定量PCR
1.
The results showed that:absolute quantitative PCR could calculate transgene copy number efficiently.
主要采用了绝对定量PCR和热不均一交错PCR(thermal asymmetric interlaced PCR,TAIL-PCR),检测了体细胞核移植技术生产的绿色荧光蛋白转基因猪中外源基因拷贝数和整合位点,并利用旁侧PCR(Junction PCR)对整合位点进行确定,同时进一步分析了整合位点的纯合性。
2)  Relative quantification of real-timePCR
PCR相对定量
3)  absolute quantification
绝对定量
1.
Absolute quantification of swine brain glutamate compounds concentration using MR spectroscopy and LCModel after nasal spraying butorphanol tartrate;
应用1~H-MRS和LCModel研究鼻喷酒石酸布托啡诺对猪脑代谢物的影响及谷氨酸复合物的绝对定量
2.
Application of inductively coupled plasma mass spectrometry(ICP-MS) in protein absolute quantification
电感偶合等离子体质谱(ICP-MS)在蛋白质绝对定量中的应用
3.
Real-time RT-PCR based on DNA subtraction for absolute quantification of gene expression in engineered lactic acid bacteria
基于DNA扣除法的Real-time RT-PCR对工程乳酸菌外源基因表达的绝对定量分析
4)  relative quantity RT-PCR
相对定量RT-PCR
1.
In order to elucidate the mechanism of its promoting growth further, effects of cyadox on the expression of IGF-1 and EGF mRNA in the primary culture piglet hepatocytes and livers were studied in this article by relative quantity RT-PCR methods.
本文运用相对定量RT-PCR方法,研究喹赛多对原代培养猪肝细胞以及仔猪肝脏组织中胰岛素样生长因子-1(Insulin-like growth factor-1,IGF-1)和表皮生长因子(Epidermal growth factor,EGF)mRNA表达的影响,探讨喹赛多抗菌促生长的机理。
5)  Real-time relative quantitative PCR
实时相对定量PCR
6)  relative quantitative real time RT-PCR
荧光实时相对定量RT-PCR
1.
In this research,the small interfering RNAs(siRNAs) obtained by in vitro transcriptional methods were applied to suppress the expression of chTERT mRNA in MDCC-MSB1 cells by relative quantitative real time RT-PCR,and to evaluate the biological consequences of chTERT down-regulation on M.
为通过体外转录的小干扰RNA(Small interference RNA,siRNA)抑制马立克氏病淋巴瘤细胞(MDCC-MSB1)中鸡端粒酶反转录酶(Chicken telomerase reverse transcriptase,chTERT)mRNA的表达,探讨chTERT在MDCC-MSB1中的作用,本试验设计了针对chTERT mRNA的特异siRNA序列(siRNA-1、siRNA-2、siRNA-3),利用T7 RNA聚合酶体外转录系统,制备21 bp的siRNA分子,通过脂质体介导转染MDCC-MSB1细胞,采用荧光实时相对定量RT-PCR方法检测转染细胞chTERT基因mRNA的相对表达量,流式细胞仪测定转染细胞周期变化情况。
补充资料:绝对
【绝对】
绝了相对,叫做“绝对”,与绝待同义。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
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