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1) hem-nested polymerase chain reaction
半巢氏PCR
1.
Objective To detect the active efflux gene qac gene in methicillin-resistant Staphylococcus aureus(MRSA) by hem-nested polymerase chain reaction(PCR) and to learn the carrier condition of qac gene.
方法:根据MRSA主动外排系统qacA和qacB基因序列设计引物,采用半巢氏PCR法对中南大学3所附属医院2006年8月至2008年3月临床分离的80株MRSA分别扩增qacA/B和qacB基因,并进行序列分析。
3) Semi-nested PCR
半巢式PCR
1.
Research on Promoter Region Methylation Status of Tumor Repressor Gene P16 in Human Hepatocellular Carcinoma by Using Methylation Sensitive Restriction Enzyme and Semi-nested PCR Assay;
限制性内切酶结合半巢式PCR法检测人肝癌P16抑癌基因启动子区甲基化研究
2.
A comparison of the detection sensitivity of five primer pairs(fOI1/rOI2c,fOI2/r23S1,fA2/rJ5,fP535/rP535,fP400/rP400)for citrus huanglongbing pathogen was carried out using simple PCR,semi-nested PCR and nested PCR.
半巢式PCR及巢式PCR的检测灵敏度远高于常规PCR,可检测出接近极限浓度10-7ng/μL,二者没有明显的差别。
3.
Semi-nested PCR method was established to detect trace ingredient of transgenic rapeseeds,which was 100 times more sensitive than the regular PCR.
建立了痕量转基因油菜籽成分的半巢式PCR(Semi-nested PCR)检测方法,灵敏度比常规PCR提高了100倍。
4) semi-nested polymerase chain reaction
半巢式PCR
1.
we detected for HPV16DNA in 152 cases of cervical carcinoma and 60 cases of normal cervical epithelia with semi-nested polymerase chain reaction.
采用半巢式PCR技术对152例维吾尔族妇女宫颈癌石蜡组织中HPV16DNA进行检测。
2.
To investigate the correlation between HPV16 infection and p53 protein expression in cervical carcinoma among Uigur women in Xinjiang,we detected for HPV16DNA and p53 protein in 80 cases of cervical carcinoma and 34 cases of normal cervical epithelia with semi-nested polymerase chain reaction and immunohistochemistry methods,respectively.
采用半巢式PCR法检测 80例新疆维吾尔族宫颈癌及 3 4例正常宫颈组织中HPV16DNA ,采用免疫组化法检测p5 3蛋白 ,分析其与宫颈癌发生的相关性 ,探讨HPV16感染和p5 3蛋白表达在新疆维吾尔族妇女宫颈癌发生中的作用。
5) SN-PCR
单管巢氏PCR
6) semi-nested RT-PCR
半巢式RT-PCR
1.
Detection of respiratory syncytial virus by semi-nested RT-PCR;
半巢式RT-PCR法检测呼吸道合胞病毒
2.
Detection of Tobacco ringspot virus and Tomato ringspot virus by semi-nested RT-PCR;
烟草环斑病毒和番茄环斑病毒的半巢式RT-PCR检测
3.
Detection of Bean pod mottle virus by semi-nested RT-PCR in imported soybean;
半巢式RT-PCR检测进口大豆中菜豆荚斑驳病毒的研究
补充资料:巢氏
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